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Evaluation of development of various strains of Beauveria bassiana in different environmental conditions
ŠEBEK, Jaroslav
The entomopathogenic fungus Beauveria bassiana is a polyphagous species of fungus that attacks more than 700 species of hosts. This species of fungus is one of the most researched and most common representatives of entomopathogenic fungi. Many of bioproducts based on conidia or blastospores of B. bassiana are registered around the world. These bioproducts are used in biological control against various pests of field crops, fast-growing vegetables and ornamental flowers and in forest stands against certain pest species. The bachelor thesis focuses on the basic "in vitro" parameters of selected strains of entomopathogenic fungus B. bassiana from different geographical areas. The work focuses on the effect of temperature on the germination of strains of the entomopathogenic fungus B. bassiana. Furthermore, the growth and development of B. bassiana strains (radial growth) were compared. And the last part is focused on the comparison of spore production of strains of entomopathogenic fungus B. bassiana on standard nutrient medium and natural substrate.
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Evaluation of qualitative and quantitative parameters of entomopathogenic fungus \kur{Beauveria bassiana} related to the inoculum production temperature
RYBAROVÁ, Kateřina
Bachelor thesis is focusing on the entomopathogenic fungus Beauveria bassiana strain GHA, which is contained in the commercial bioproduct Botanigard. Botanigard is a product used in biological control in many countries around the world to control a wide range of pests of various plant species. In this thesis, "in vitro" (radial growth, spore production) and "in vivo" parameters (virulence) of the GHA strain of Beauveria bassiana were tested at various temperatures on different nutrient media. The most optimal temperature for radial growth of the GHA strain was 25 °C, on media with a nitrogen to carbon ratio of 20:20. The GHA strain produced the largest amount of conidia on the medium with a nitrogen/carbon ratio of 20:40 at temperature 25 °C. On the contrary, the smallest conidia production was obtained on medium PDA and nitrogen/carbon medium in ratio 5:10. The temperature of 25 °C was also the most optimal for the infection of larvae of Tenebrio molitor. Thesis also deals with the comparison of blastospores production of GHA strain with other strains of B. bassiana isolated from different geographical areas. The strain isolated in Argentina had the highest production of blastospores on MEB medium.
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Vliv teploty na vývoj a virulenci entomopatogenní houby Metarhizium anisopliae
SANDALA, DAVID
Abstract Entomopathogenic fungus M. brunneum has a high potential in integrated pest management against targeted pests. The commercial bio-product Met52 is based on the strain F52 of entomopathogenic fungus M. brunneum. Temperature is the key factor in the inoculums production of strain F52. The optimal temperature for the cultivation of M. brunneum strain F52 is 25 °C. However, the influence of the temperature of inoculum production plays an important role on the growth and spore production during the subsequent cultivation in different temperature conditions. The optimal temperature for radial grows of strain F52 of M. brunneum from different inoculums is 25 °C and subsequently 30 °C. The cultivation of inoculums at 10 °C appears to be negative because at this temperature the strain grows very slow, and spore production is minimal. The morphology phenotype change of inoculums is influenced by 30 °C of cultivation and also this temperature affects the appearance of inoculum central colonies. The highest production of spores was determined from the colonies established from all inoculums cultivated at 25 °C. The highest mortality and the greatest fungus development was recorded at 25 °C and the most virulent to larvae T. molitor were inoculums produced at 15 °C and 20 °C.
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Vzájemná kompatibilita entomopatogenní houby \kur{Isaria fumosorosea} s dalšími druhy entomopatogenních hub
OUŠKOVÁ, Šárka
This thesis is focused on evaluation of the compatibility of entomopathogenic fungus Isaria fumosorosea with different species of entomopathogenic fungi and mycoparasitic fungi at different temperatures. The strains of species Beauveria bassiana, Metarhizium anisopliae, Lecanicillium muscarium, Coniothyrium minitans and Clonostachys rosea f. catenulata were selected for experiments base on compatibility. The results showed that combination of I. fumosorosea with species L. muscarium is compatible. The species do not limit to each other in the environment at all temperatures (15, 23 and 25 °C). On the other side, fungus I. fumosorosea in combination with other species have affected their growth and spore production. The efficacy of entomopathogenic fungi against larvae Tenebrio molitor was evaluated. The most effective species against larvae were species I. fumosorosea, B. bassiana and M. anisopliae. On the contrary, the smallest effective was observed after infection larvae by L. muscarium. Mycoparasitic fungus C. rosea f. catenulata was not able to directly infect larvae of T. molitor. This species did not infect healthy larvae. However it is able to infect weakened individuals or is growing as saprotrophs on the cadavers.
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